Composite

Part:BBa_K5048017

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-13)


pBAD-Lpp'OmpA-DDDDK-QEP-GST-dt

This part is the surface-decorating system which display small peptides QEP in the outer leaflet of the outer-membrane in E.coli. The DDDDK enterokinase cleavage site can be recognized by enteroskinase and small peptides will be cut off. It contains the araBAD promoter, Lpp'OmpA(codon optimised), enterokinase site, QEP, GST tag, rrnB T1 terminator and T7Te terminator.It is used in the verification of the promoter-changing cycle.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
    Illegal AgeI site found at 655
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56
    Illegal SapI.rc site found at 837


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Categories
Parameters
None